Bio-Rad C1000 Thermal Cycler
Thermal cyclers, also known as PCR machines, regulate the temperature of polymerase chain reaction. This allows for quick heat exchange and amplification of target DNA sequences. Discover the history of this technology and advances in PCR machine design.
The results from this study showed that a fast protocol able to produce high quality profiles can be achieved in 26 min with SpeedSTAR HS DNA polymerase and a Bio-Rad C1000 Touch thermal cycler.
Easy to use
The CFX96 system builds on the Bio-Rad C1000 thermal cycler by adding an interchangeable optical reaction module to make it a powerful real-time PCR detection system. The optical system features a fixed array of 48 long-lasting LEDs that illuminate sample wells sequentially and emit light into a photodiode for sensitive detection. This innovative design eliminates cross talk and maximizes fluorophore excitation for optimal multiplex detection.
The large graphical display makes it easy to set up runs and monitor amplification traces. Plus, the Protocol Autowriter helps you create your optimal qPCR protocol in just three clicks or less.
CFX Manager software delivers quick and easy run setup with advanced gene expression data grouping tools (bar chart, clustergram, scatter plot, volcano plate). Using CFX Manager software, users can also easily analyze results to gain insights from your experiments.
Fast ramping
With fast ramping capabilities, the bio-rad c1000 thermal cycler offers a convenient user interface. The device also allows users to store data on a USB flash drive. It has a wide range of other features that make it easy to use.
A high-quality Identifiler profile can be generated in 26 min using a combination of fast PCR protocols, SpeedSTAR HS DNA polymerase and the Bio-Rad C1000 thermal cycler. This is a significant reduction in cycle time, especially for cases where DNA analysis needs to be completed quickly (e.g., suspect DNA profiling before a detention period expires).
The system also has an intelligent design that ensures optimum performance and durability. This includes a robust temperature control, which eliminates cycling-related errors and ensures the accuracy of profiles.
Easy to program
The c1000 thermal cycler has an intuitive, large color touch screen that lets you create a protocol in three easy steps and save it in your personal folder. You can also run a protocol that was automatically written by Protocol Autowriter. These features help you save time and avoid error.
The system has six interchangeable reaction modules that meet your throughput needs. These include a 96-deep well block that holds 0.2 and 0.5 ml tubes, a 96-well fast module for high throughput, a dual 48-well fast module, and a real-time PCR detection module. You can swap the blocks in less than 15 seconds without tools.
The programmable temperature gradient allows you to identify the optimal annealing temperature for a reaction in a single run, which saves you time. And a power-save mode reduces energy consumption and environmental impact when the instrument is not in use.
Optical reaction module
Thermal cyclers use Peltier elements to achieve fast and precise temperature changes. They often have blocks with holes where reaction tubes can be insert to heated and cooled in discrete, pre-programmed steps.
The LED-driven optical cavity PCR has a dual layer of thin Au films to evenly absorb light, significantly improving PCR mixture temperature uniformity. It also has comparable cycling performance to other commercially available POC thermal cyclers.
Easily configure the c1000 for different throughput needs with five interchangeable reaction modules that swap in seconds without tools. Save time with the built-in library of effective methods for qPCR and real-time RT-PCR applications. And simplify programming with streamlined graphics. The c1000 offers all the features you need to advance your qPCR workflows.
Powerful real-time PCR
The c1000 thermal cycler packs a lot of functionality into a small package. Its efficient touch-screen navigation makes it easy to create a protocol, start a run, or manage files. The programmable temperature gradient function helps you identify the optimal annealing temperature in a single run, saving time and optimizing results.
The system uses a combination of reverse transcriptase and DNA polymerase to produce cDNA molecules that match target sequences. These cDNA molecules are then amplified using specific primer and probe sets. The fluorescence intensity of the resulting products is measurand report as an indication of the assay result.
The CFX96 real-time system adapts to your workflow by offering a fully modular platform that accommodates different throughput needs with easily interchangeable modules that swap in seconds without tools. These electrically heated and cooled modules maintain precise control, even during ramping, to ensure consistent PCR results.
