Bio-Rad C1000 Thermal Cycler

Forensic STR genotyping protocols often require long PCR cycling times which significantly slow down DNA sample processing. The goal of this study was to develop a fast protocol that produces complete and interpretable Identifiler profiles in 26 min. This was achieved by replacing AmpliTaq Gold with SpeedSTAR HS DNA polymerase, modifying the cycling parameters (shortened hold times at each temperature step), and adopting a two-step PCR approach.

Ease of Use

Designed with simplicity in mind, the bio rad c1000 thermal cycler makes it easy to set up and use. Integrated controls provide quick and simple operation, and a large color touch screen with intuitive navigation allows for easy programming. The protocol autowriter feature helps you create your own protocols in as few as three steps, saving time and effort.

PCR reactions were performed as describ in the product manual with the ARM-D for b-Lactamase ID kit (Applied Biosystems). Amplification was monitored on a 2.5% agarose gel. PCR amplicons were detected with a Molecular Imager Gel Doc EZ System and visualized by digital imaging software.

The c1000 touch provides exceptional thermal performance in a flexible design, providing you with a full range of PCR throughput options. Each instrument features interchangeable reaction blocks that switched out in seconds without tools. Each block has an adjustable heated lid to support a wide range of vessels and sealers, including low-profile plates. You can even daisy chain up to four instruments together and control them through a single PC for high-throughput applications.

Durability

Designed to last, this PCR cycler uses thermal gradient technology to adjust temperatures during a PCR run. It is compact, efficient and easy to use. Its intuitive touch screen saves time programming, and nonvolatile memory preserves data during power outages. The device also allows users to easily store protocols in custom folders and USB flash drives.

Depending on your needs, you can choose from a variety of plate configurations. There are fix-block models that allow only a single block configuration, while others feature interchangeable heads with desirable configurations. This means that you can get the functionality of a high-end PCR machine at a much more affordable price.

This PCR system use to perform a b-lactamase ID assay for antibiotic resistance. The ARM-D for b-lactamase identification kit is used to detect antibiotic resistance in Gram-negative bacteria. The amplicons are then stain with ethidium bromide and resolved on a 2.5% agarose gel. The results are then analyzed with Bio-Rad Gel Doc EZ System software.

Convenience

The Bio-Rad c1000 thermal cycler packs an efficient package of user friendly functions into a small footprint. The intuitive touch screen makes it easy to create a protocol and start a run, which helps save lab time. It also provides convenient storage of multiple protocols and settings.

This model equipped with a CFX96 optical reaction module to transform it into a real-time PCR detection system. The solid-state optical system illuminates each sample with an array of 48 long-lasting blue-green LEDs, minimizing cross talk and maximizing fluorescence excitation for successful detection. The results are then read filter photodiodes to identify the target sequences with high sensitiv.

Using the CFX Manager software, the system provides simple experiment setup and analysis for both real-time qPCR and conventional PCR. The software also supports multiple instrument control for flexibility and offers tools to help meet US FDA 21 CFR Part 11 compliance. This advanced software makes it easier than ever to achieve reliable, robust PCR results.

Performance

Using dynamic ramping technology, the C1000 Touch PCR thermal cycler quickly checks multiple temperature settings and finds an optimal annealing point. This feature saves you time and ensures consistency in every PCR run, year after year.

This PCR machine accommodates different throughput needs with easily interchangeable 96-well fast reaction modules that swap in seconds without tools. Each PCR module can handle multiple PCR assays in both plate and tube formats.

These PCR machines feature a large color touch screen that makes it easy to create a protocol and start a run, saving you time programming. Dedicated folders keep your protocols organized so you can easily find and edit them.

Adding the optional CFX96 optical reaction module transforms this PCR machine into a powerful real-time PCR system. A fixed array of 48 long-lasting blue-green LEDs illuminates samples sequentially and filtered photodiodes detect fluorescence, minimizing cross talk and maximizing signal detection for sensitive, accurate results. Combined with the flexible CFX Manager software, this real-time PCR solution offers sophisticated qPCR tools and techniques within an affordable compact platform.